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dc.contributor.authorAboy, Ana Lúcia
dc.contributor.authorAnders Apel, Miriam
dc.contributor.authorDebenedetti, Sílvia
dc.contributor.authorFrancescato, Leandro
dc.contributor.authorRosella, María Adelaida
dc.contributor.authorHenriques, Amélia Teresinha
dc.date.accessioned2014-07-24T18:13:38Z
dc.date.available2014-07-24T18:13:38Z
dc.date.issued2012
dc.identifier.urihttp://repositorio.ub.edu.ar/handle/123456789/2708
dc.description.abstractBaccharis trimera commonly named ‘carqueja’, is wide-spread in South America and are used as raw material for herbal medicines. A reversed-phase liquid chromatography (RP-LC) method coupled to diode array detector was developed for the analysis of caffeoylquinic acids (CQAs), the main compounds responsible for its digestive activity. The identity of the quinic acids was established by mass spectrometry and were them: 5-O-[E]-caffeoylquinic acid, 3,4-O-[E]-dicaffeoylquinic acid, 3,5-O-[E]-dicaffeoylquinic acid, 4,5-O-[E]-dicaffeoylquinic acid and a tricaffeoylquinic acid. The RP-LC method for the quantitation of the caffeoylquinic acids was validated according to ICH guidelines, based on the following parameters: linearity, selectivity, robustness, limits of detection and quantification, precision and recovery. Hydroalcoholic extracts were prepared by the maceration of the plant material with ethanol:water 1:1 (v/v) in a 0.1:25 g mL−1 plant:solvent ratio in a water bath at 40 °C. Validation data indicated that the HPLC method proposed is suitable for the analysis of caffeoylquinic acids in B. trimera raw material. The results of the LOD and LOQ analyses for the 5-CQA were 4.1 μg mL−1 and 12.5 μg mL−1, respectively, 1.3 μg mL−1, 3.9 μg mL−1 for 4,5-diCQA and 1.7 μg mL−1, 5.1 μg mL−1 for triCQA. The levels of total CQAs ranged from 2.1 to 4.0 g% (w/w). The influence of season harvest and site collection was also evaluated and variations were observed in the results and can be related to phonologic phase, different locations, seasons and soil. Long term and photostability of plant material were carried out and was observed a stable behavior during the time of the experiments.es_ES
dc.language.isoenes_ES
dc.publisher.EditorUniversidad de Belgrano. Facultad de Ciencias Exactas y Naturales. Proyectos de Investigación
dc.relation.ispartofseriesA Journal of Chromatography;Volume 1219, 6 January 2012, Pages 147–153
dc.subjectBaccharis trimeraes_ES
dc.subjectCaffeoylquinic acidses_ES
dc.subjectDicaffeoylquinic acidses_ES
dc.subjectTricaffeoylquinic acidses_ES
dc.subjectRP-LCes_ES
dc.subjectPhotostabilityes_ES
dc.subjectFotoestabilidad
dc.titleAssay of caffeoylquinic acids in Baccharis trimera by reversed-phase liquid chromatographyes_ES
dc.typeArticlees_ES


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